Search results for " dynamic binding capacity"
Article
How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
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In protein purification, dynamic binding capacity (DBC) of a chromatography column describes the maximum amount of target protein that you can load onto your co…
Article
Determining and Optimizing Dynamic Binding Capacity
Dynamic binding capacity (DBC) indicates the quantity of a specific biomolecule that can be bound during a chromatography purification step under a specific set of process conditions. Determ…
Article
Dynamic Binding Capacity in Fiber-Based Chromatography
Just like with resins, the dynamic binding capacity (DBC) of a Fibro unit is affected by process conditions and by the properties of the target molecule. Thus, determining the DBC of a Fibro unit is s…
Poster
Optimizing dynamic binding capacity and aggregate clearance in an mAb polishing step
Capto™ S ImpAct is a strong cation exchange (CIEX) chromatography medium (resin) designed for monoclonal antibody (MAb) polishing steps. In this study, optimization of binding conditions targeting h…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
Optimizing binding capacity
The dynamic binding capacity is dependent upon several experimental parameters (e.g., the charge and size of the target molecule, pore size, and the charge of the chrom…
Article
Re-use of Protein A Resin: Fouling and Economics
Recent innovations in Protein A-affinity chromatography have targeted creation of new resins capable of delivering high dynamic binding capacity and high throughput with better mass-transfer propertie…
Article
Improving Process-Scale Chromatography
…of further improving process economy and robustness in mAb manufacturing processes by increasing the dynamic binding capacity and alkali stability of Protein A resins.
For other chromatography st…
Article
Enhancing Resins Addresses Purification Concerns
“As upstream processing continues to increase titers of these biologic molecules, improving on current chromatography resins to increase the dynamic binding capacity while enhancing separation to remo…
Article
Evolution of the Monoclonal Antibody Purification Platform
The authors discuss the evolution of the purification platform for manufacturing of mAb therapeutics.
Monoclonal antibodies (mAb) increasingly form the majority share of the product pipeline…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
A novel prototype Resin 3 offers an increased dynamic binding capacity (DBC) at a slightly longer residence time.
Evaluating capacity and reusability
Table I shows the Protein A resins that we…